Elisa Reader And Washer Principle

And a plate reader software is used to plot standard curves and calculate results.

Elisa reader and washer principle.

A microplate washer is used to wash away non specific material in the wells and an absorbance elisa microplate reader detects the color change produced when target antigen is present. Readers need to have appropriate filter 650 nm and 450 nm. Having the reactants of the elisa immobilized to the microplate surface enables easy separation of bound from non bound material during the assay. This ability to wash away non specifically bound materials makes the elisa a powerful tool for measuring specific analytes within a crude preparation.

The principle in elisa various antigen antibody combinations are used always including an enzyme labeled antigen or antibody and enzyme activity is measured colorimetrically. The enzyme activity is measured using a substrate that changes color when modified by the enzyme. An immune reaction i e. It can be manual system that washes one row or column at a time or semi automated systems that wash one strip or plate at a time or fully automated systems that can process multiple plates.

Are available as fixed as well as adjustable volume as well as single channel and multi channel. The elisa reader reads the plate. In short it is an antibody test or a test for immune response to things attacking the body such as virus bacteria and allergens. The illustration below shows a workflow for a typical sandwich elisa assay.

Along with the enzyme labelling of antigens or antibodies the technique involves following three principles in combination which make it one of the most specific and sensitive than other immunoassays to detect the biological molecule. What an elisa reader does.